What does pen strep do?
What does pen strep do?
Penicillin-streptomycin (Pen-strep) is a common antibiotic used to prevent bacterial infection in cell culture and clinical treatment.
How much pen strep do you add?
Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 µg/mL streptomycin.
What is 1x pen strep?
Penicillin Streptomycin solution, also known as Pen Strep, is the most commonly used antibiotic solution for the culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.
Where do you inject a Pen with strep?
administering 1 ml Pen & Strep per 25 kg bodyweight. The dose should be given once daily by deep intramuscular injection for up to three consecutive days. The maximum dose volume administered at one site should not exceed 15 ml for horses, 6 ml for cattle, 3 ml for sheep and 1.5 ml for pigs.
What is the withdrawal period for Pen-Strep?
Animals must not be slaughtered for human consumption during treatment. Cattle intended for human consumption should not be slaughtered until 23 days after the last treatment. Pigs intended for human consumption should not be slaughtered until 18 days after the last treatment.
How long is Pen-Strep stable at 4c?
Overview
| Storage and Shipping Information | |
|---|---|
| Storage | +2°C to +8°C |
| Do not freeze | Ok to freeze |
| Special Instructions | Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at -20°C or for up to 2 weeks at 4°C. |
Why glutamine is important in cell culture?
Primary Functions of Glutamine in Cell Culture Systems Glutamine supports the growth of cells that have high energy demands and synthesize large amounts of proteins and nucleic acids. It is an alternative energy source for rapidly dividing cells and cells that use glucose inefficiently.
Can you give Pen-Strep to dogs?
Cautions. Penicillin is generally considered safe for use in dogs, cats, horses, livestock, and many exotic pets. It can cause a disruption of the normal bacterial population within the gut of some species, especially rodents.