What is intravital fluorescence microscopy?

What is intravital fluorescence microscopy?

Intravital microscopy is a powerful imaging tool for visualizing various biological processes, simultaneously. This allows for in vivo study of several critical cell types that can be difficult to fully understand when present in cell cultures, such as neurons or tumor cells.

What does intravital?

Definition of intravital 1 : performed upon or found in a living subject. 2 : having or utilizing the property of staining cells within a living body — compare supravital.

What is multiphoton imaging?

Multiphoton microscopy is a powerful tool for visualizing cellular and subcellular events within living tissue with its inherent “optical sectioning” capability, deeper penetration and minimal phototoxicity and photobleaching. Multiphoton microscopy can capture whole organisms or embryos on a large scale.

What is microscopy in biology?

Microscopy is the technical field of using microscopes to view samples & objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye).

What is the function of multiphoton excitation microscope?

Multiphoton excitation microscopy provides high axial (optical sectioning) and lateral resolution, reduced out of focus photobleaching/photodamage, and is optimal for deep tissue imaging (Helmchen & Denk, 2005).

What is multiphoton fluorescence microscopy?

Multiphoton fluorescence microscopy is a powerful research tool that combines the advanced optical techniques of laser scanning microscopy with long wavelength multiphoton fluorescence excitation to capture high-resolution, three-dimensional images of specimens tagged with highly specific fluorophores.

What is the application of confocal microscopy?

Applications of confocal microscopy in the biomedical sciences include the imaging of the spatial distribution of macromolecules in either fixed or living cells, the automated collection of 3D data, the imaging of multiple labeled specimens and the measurement of physiological events in living cells.

What is microscopy and its application?

Microscopy is the act of using a microscope to view tiny things that cannot be seen with the unaided eye. There are three main types: optical microscopy, scanning probe microscopy, and electron microscopy. Optical microscopes bounce light up objects and use lenses or mirrors to magnify the image.