What is Pap stain in cytology?
What is Pap stain in cytology?
The Papanicolaou (PAP) Stain Kit (Cytology Stain) is designed to differentiate between a variety of cells in vaginal smears for detection of vaginal, uterine and cervical cancer. In addition, this procedure is valuable for staining a variety of other bodily secretions and cell smears.
What staining is done in cervical cytology?
Papanicolaou stain (also Papanicolaou’s stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
How do you stain a cytology?
Here are some tips for this procedure:
- Make sure your sample on the slide is dry.
- Stain only one slide. Always submit 2-4 unstained slides with the stained slide.
- Don’t stain the best slide.
- Stain cytology slides in uncontaminated jars. Separate these jars from the stain used for infected samples, such as ear cytology.
What fixation is used for Pap cytological staining?
Rapid fixation in alcohol (wet version) is essential for pap staining, which brings out nuclear details clearly, allowing better identification of malignant cells.
Which stain is most commonly used in cytology?
Papanicolaou’s stain
Papanicolaou’s stain (PAP stain) is the most important stain in cytology and is used in all cervical smear and non-gynaecologic exfoliative smears.
What is the most critical step in the PAP staining?
As a limitation of this study, we should mention that UF-PAP stain is very sensitive technique, thus air-drying is a critical step. Also, due to the omission of O-G-6 in UF-PAP method, this method cannot be used for the diagnosis of squamous cell carcinoma.
What are the advantages of PAP stain?
The advantages offered by this staining method are the following: (1) The epithelial cells and the erythrocytes are more transparent. Overlapping cells can be more easily differentiated. (2) The color of the acidophilic cells varies from red to orange. This helps in the identification of certain smear types.
How do you stain a Pap smear?
Basic Procedure for Papanicolaou Staining (Pap stain)
- Fix the smear with 95% Ethanol 15 minutes.
- Rinse in tap water.
- Add the Harris Hematoxylin dye for 1-3 minutes.
- Rinse in tap water or Scott’s tap water.
- Dip the preparation in 95% Ethanol 10 dips.
- Add orange G-6 stain for 1.5 minutes.
- Dip in 95% Ethanol 10 dips.
What is the principle of PAP staining technique?
The principle of pap stain is to clearly distinguish between basophilic and acidophilic cell components and obtain a detailed chromatin pattern. The pap stain has 3 solutions having 6 dyes.
Why Pap stain is done?
Pap staining is used to differentiate cells in smear preparations of various bodily secretions; the specimens can be gynecological smears (Pap smears), sputum, brushings, washings, urine, cerebrospinal fluid, abdominal fluid, pleural fluid, synovial fluid, seminal fluid, fine needle aspiration material, tumor touch …
How do you use Pap stain?
What is the most critical step in the PAP staining Why?