What is the antisense technology?
What is the antisense technology?
Antisense technology is when a piece of RNA that is complementary in sequence is used to stop expression of a specific gene. Antisense therapy is a form of treatment for genetic disorders or infections.
How do antisense oligonucleotides work?
Small pieces of DNA or RNA that can bind to specific molecules of RNA. This blocks the ability of the RNA to make a protein or work in other ways. Antisense oligonucleotides may be used to block the production of proteins needed for cell growth.
Who discovered antisense technology?
Introduction. The potential of oligodeoxynucleotides to act as antisense agents that inhibit viral replication in cell culture was discovered by Zamecnik and Stephenson in 1978 [[1]]. Since then antisense technology has been developed as a powerful tool for target validation and therapeutic purposes.
How do antisense drugs work?
Antisense drug: A medication containing part of the non-coding strand of messenger RNA (mRNA), a key molecule involved in the translation of DNA into protein. Antisense drugs hybridize with and inactivate mRNA. This stops a particular gene from producing the protein for which it holds the recipe.
What is the purpose of RNA interference?
RNA interference is a natural process with a role in the regulation of protein synthesis and in immunity. It’s also a potent tool for the exploration and manipulation of gene expression.
What are antisense oligos?
Antisense oligonucleotides (ASOs) are short, synthetic, chemically modified chains of nucleotides that have the potential to target any gene product of interest. Typically, an ASO is a single-stranded sequence complementary to the sequence of the target gene’s transcribed messenger RNA (mRNA) within a cell.
What is the difference between Sirna and ASO?
Both platforms are intended to modulate gene expression. Both are nucleic acids and contain an antisense strand intended to recognize a target mRNA. They also have important differences. ASOs have one strand while siRNAs have two, a basic fact that may lower cost and simplify delivery.
What is the difference between antisense and RNAi?
Definition. Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. In contrast, RNA interference (RNAi) is triggered by double-stranded RNA (dsRNA) and causes sequence-specific mRNA degradation of single-stranded target RNAs in response to dsRNA.