What does WGA stain for?
What does WGA stain for?
Wheat germ agglutinin (WGA), which binds to glycoproteins of the cell membrane, is routinely used for the staining of skeletal and cardiac sarcolemma to determine cross sectional area or myocyte density. In this context we observed that WGA also labeled fibrotic tissue in cardiac sections after myocardial infarction.
How does hypertrophy lead to fibrosis?
This is usually referred to as the compensatory phase of cardiac hypertrophy and enhanced collagen deposition in this stage may be beneficial as the collagen matrix supports the increase in muscle mass. Conversely, more severe pressure overload for a prolonged period leads to excessive collagen deposition or fibrosis.
What is extensive myocardial fibrosis?
Myocardial fibrosis is defined as an increased quantity of collagenous scar tissue in the heart. Myocardial fibrosis may arise as a result of cardiac disease and/or extracardiac diseases. Myocardial fibrosis is associated with an unfavourable prognosis.
Does cardiac tissue heal by fibrosis?
Cardiac fibrosis is a process of pathological extracellular matrix (ECM) remodeling, leading to abnormalities in matrix composition and quality, as well as an impaired heart muscle function . Initially, ECM deposition is a protective mechanism and can be beneficial for wound healing and tissue regeneration.
Where is WGA found?
WGA is a protein found abundantly in wheat kernels. It has a high binding affinity for N-acetyl-glucosamine (GlcNAc), one of the essential components of PG glycan strands, and thus can be used to probe PG specifically.
Is WGA cell permeable?
PhenoVue Fluor 488 – and 594 – WGA are not cell-permeable, therefore fixed but non-permeabilized cells exhibit plasma membrane staining, whereas additional permeabilization step enables staining of cytoplasmic membranes such as Golgi apparatus.
Does ischemia cause fibrosis?
Cardiac Fibrosis as a Consequence of Ischemic Heart Disease A variety of hemodynamic, toxic, metabolic, and immunologic disturbances trigger fibrotic remodeling of the myocardium. In patients with chronic IHD myocardial infarcts result in replacement of dead cardiomyocytes with collagen-based scar.
What foods contain WGA?
Lectin activity has been demonstrated in wheat, rye, barley, oats, corn and rice, however the best studied of the cereal grain lectins is WGA . The highest WGA concentrations are found in wheat germ (up to 0.5 g/kg ).
Is WGA-FITC a suitable marker for cardiac fibrosis in frozen tissue sections?
The fibrosis amount was quantified in a histogram-based approach using the non-commercial image processing program ImageJ. Our results clearly demonstrate that WGA-FITC is a suitable marker for cardiac fibrosis in frozen tissue sections.
Is wheat germ agglutinin staining a suitable method for detecting fibrosis in cardiac tissue?
Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction. B Emde Heinrich-Heine University Düsseldorf.
Can WGA label fibrotic tissue after myocardial infarction?
In this context we observed that WGA also labeled fibrotic tissue in cardiac sections after myocardial infarction. Thus, we systematically analyzed in this study WGAs potential for the staining and quantification of post MI scar formation.
What is the best staining method for fibrosis?
A typical fibrosis staining method is the picrosirius red stain.3A couple of years ago, Gaspard and Pasumarthi4published a color-subtractive computer assisted image analysis (CS-CAIA)-based quantification method for diffuse fibrosis on sirius red/fast green stained tissue sections.